EN ISO 21420 is applicable and required for all PPE gloves. It defines the general rules for comfort, innocuousness, marking, packaging and user notice. The EN ISO 21420 will replace the EN 420:2003 + 1:2009 when EN ISO 21420 will be published in the Official Journal of the European Union. From a standardization point of view and for the CEN, EN 420 will be deleted after September 30th, 2020.

The list of substances to check
· pH of all materials now including rubber and plastic. Each material shall be tested separately.
· Chromium VI in leather and each leather type and each colours shall be tested separately
· Azo colorants which release carcinogenic amines
· DMFa (dimethylformamide) in glove containing PU (1 000 mg/kg)
· PAH in rubber or plastic materials in direct contact with skin (1 mg/kg) whether the glove is intended for private or professional use
· Nickel in metallic parts in prolonged contact with skin

Marking (glove or packaging)
· Glove & Size designation · The CE marking · Relevant pictograms and corresponding level(s) of protection
· Date of manufacturing (month and year) · Manufacturer’s name and postal address
* If marking on glove is not possible, due to the characteristics of the product then the marking shall be affixed to the first packaging enclosure.

The Framework Regulation (EC) 1935/2004 states that food contact materials shall be safe. They shall not transfer their components into the food in quantities that could endanger human health, change the composition of the food in an unacceptable way or deteriorate the taste and odour of foodstuffs.
EU No 10/2011 is applicable regulation for all the Food contact material or Article made up of Plastics.
The (EU) No. 10/2011 d introduces separate rules for testing conditions for OML and SML testing.
The analytical and technical description of migration tests are established in the following EN standards:
• Several parts of the EN1186 standards describe the testing procedure for overall migration testing.
• Several parts of the EN13130 standards describe the general testing procedure for specific migration testing
including the analytical measurement of about 26 substances.

The American National Standards Institute (ANSI) has released a new edition of the ANSI/ISEA 105 standard – 2016. The changes include new classification levels, which includes a new scale to determine the ANSI cut score and a revised method for testing gloves to the standard.
The new ANSI standard features nine cut levels that reduces the gaps between each level and better defines protection levels for the cut resistant gloves and sleeves with the highest gram scores.